KCL009-trans-1

KCL009/trans-1

Cell Line Name KCL009/trans-1
Cell Line Status Currently available
Cell Type Human Embryonic
Grade Research
Source Human embryo / blastocyst
Source type Fresh
Consent Status Donor consent was reviewed by the UK Steering Committee and was found to be consistent with the Steering Committee Code of Practice for the Use of Human Stem Cell Lines.  
Date cell line derived 19 April 2007
Ethical Statement In the opinion of the Steering Committee for the UK Stem Cell Bank and the use of Human Stem Cell Lines, the derivation of the stem cell line is consistent with UK ethical guidelines
Depositor Kings College London
Depositing Centre Kings College London
Country of Origin United Kingdom
MUL Contact Details  paul.labbett@kcl.ac.uk 
Journal Reference Publications available
SCSC No. SCSC-07-49(2)
UKSCB Accession No. R-08-011
Distribution Bank No. D01  
Passage No. p12
Feeder Cell Type Mouse embryo fibroblast
Basel Medium KO-DMEM
Protein Source KO-SR
Growth Factors bFGF
Incubation Conditions 37°C, 5% CO2, >95% RH, Subculture: Enzyme subculture  
Culture Morphology Embryonic stem cell colonial morphology
Preservation Method freezing (10% DMSO)  
Viability >90%
DNA Profile Human
Karyotype 46, XX - mixed karyotype population
Characterisation Panel Positive for pluripotency markers
Sterility Test No contamination detected by EU Pharmacopeia Sterility Test
Mycoplasm Screen Mycoplasma not detected by Mycoplasma PCR or Culture
Viral Screen Human Virus Panel (HIV, HTLV, HBV, HCV, EBV, CMV) not detected by PCR

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